![]() Roughening both the PDMS stamp and substrate resulted in up to a 20-fold improvement in the transfer of BSA-Alexa Fluor 647 from the stamp to the substrate. Surface roughening with the particle slurry also improved the ability to stamp molecules onto the substrate during microcontact printing. Cell adhesion to the roughened surfaces was also increased compared to native surfaces. The quantity of adherent fibronectin was increased on roughened arrays by two-fold over that on native arrays. Native and roughened arrays were assessed for their ability to support fibronectin adhesion and cell attachment and growth. Less than 0.8% of the microstructures on the array were removed or damaged after 5 min of polishing. ![]() This represented a 4−9 fold increase in surface roughness relative to that of the native surface. A maximal RMS roughness of 7−18 nm for the surfaces was obtained within 15−30 s of polishing with the slurry. The roughness and the percentage of dislodged or damaged microstructures was evaluated as a function of the roughening time for both SUF structures. SU8, 1002F, and polydimethylsiloxane (PDMS) microstructures were roughened by polishing with a particle slurry. A clamping mechanism according to claim 3, wherein said cylindrical case has a female taper with an inner diameter thereof increasing toward outside at one end confronting the pull stud of the workpiece-mounting pallet so that a tapered cone having the pull stud at a distal end thereof is fitted into said female taper.A method to perform in situ roughening of arrays of microstructures weakly adherent to an underlying substrate was presented. ![]() ![]() A clamping mechanism according to claim 3, further comprising a drive mechanism for moving said eject bar with respect to said cylindrical case in a direction of conveying the workpiece-mounting pallet from the work table by utilizing movement of the work table, wherein said drive mechanism moves said eject bar to press the pull stud of the workpiece-mounting pallet in the direction of conveying the workpiece-mounting pallet from the work table against the urging force of said elastic member so that the workpiece-mounting pallet is unclamped. A clamping mechanism for clamping/unclamping a workpiece-mounting pallet on a work table of a machine tool in a pallet changer for changing workpiece-mounting pallets, comprising:Ī sleeve slidably supported in said cylindrical case Īn eject bar arranged inside of said sleeve and movable with said sleeve Īn elastic member generating an elastic force for urging said eject bar and said sleeve with respect to said cylindrical case in a direction of conveying the workpiece-mounting pallet to the work table andīalls arranged movable radially of said cylindrical case to be in contact with an inner peripheral portion of said sleeve, wherein said sleeve slides in said cylindrical case by the elastic force of said elastic member such that said balls are moved radially inward of said cylindrical case to retain a pull stud provided on the workpiece-mounting pallet to be unmovable in the direction of conveying the workpiece-mounting pallet to/from the work table, thereby the workpiece-mounting pallet is clamped on the work table. A clamping mechanism according to claim 1, wherein the workpiece-mounting pallet is unclamped by utilizing a drive force of a driving mechanism of the machine tool provided for axially moving the work table. supporting means for supporting the workpiece-mounting pallet movable in a direction of conveying the workpiece-mounting pallet to and from the work table andĬlamping means for clamping/unclamping the workpiece-mounting pallet in the direction of conveying the workpiece-mounting pallet supported by said supporting means, wherein the workpiece-mounting pallet is moved on a plane parallel to the work table and clamped/unclamped on the work table.A clamping mechanism for clamping/unclamping a workpiece-mounting pallet on a work table of a machine tool in a pallet changer for changing workpiece-mounting pallets, comprising:
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